Aaron Connolly ended the ePremier League Invitational defence of Wolverhampton Wanderers as the Brighton & Hove Albion striker defeated Morgan Gibbs-White in the second edition of the tournament.
Wolves were defending their crown after Diogo Jota won last month's inaugural tournament but Connolly claimed a 3-0 victory on Wednesday to take Brighton into the quarter-finals.
Earlier in the day, Keinan Davis put Aston Villa into the last eight with a 6-1 victory over former Liverpool defender Jose Enrique.
Southampton's Michael Obafemi also made it through with an entertaining 7-0 triumph over former boxer Tony Bellew, representing Everton.
In the final contest, Chelsea wing-back Emerson was too strong for Watford forward Andre Gray, whom he beat 4-2.
Day 2 results
Jose Enrique (LIV) 1-6 Keinan Davis (AVL) | Report and highlights
Morgan Gibbs-White (WOL) 0-3 Aaron Connolly (BHA) | Report and highlights
Tony Bellew (EVE) 0-7 Michael Obafemi (SOU) | Report and highlights
Emerson (CHE) 4-2 Andre Gray (WAT) | Report and highlights
The last 16 continues on Thursday with four more matches.
Day 3 matches
James Maddison (LEI) 8-1 Callum Wilson (BOU)* | Report and highlights
Max Aarons (NOR) 4-1 Ryan Sessegnon (TOT) | Report and highlights
Phil Foden (MCI) 3-4 Charlie Taylor (BUR) | Report and highlights
John Egan (SHU) 4-3 Jofra Archer (MUN) | Report and highlights
James Maddison (LEI) 3-2 Max Meyer (CRY) | Report and highlights
* Round 1 fixture
See: ePL Day 1 recap
See: ePL Invitational draw
How to watch the tournament
Fans can watch all the action on the Premier League's digital channels as well as on the Sky Sports YouTube and Twitch channels, BBC Sport website and iPlayer.
Finals Day will also be shown live in the UK on Sky Sports' Premier League and Main Event channels, with the tournament again being televised around the world by the Premier League's international broadcast partners.
The tournament will urge everyone to follow official advice by staying at home and saving lives.
We would also ask anyone inspired to play FIFA 20 to take regular breaks from gaming, every 45 minutes.